Selective Binding of Endostatin Peptide 4 to Recombinant VEGF Receptor 3 In Vitro.
Identifieur interne : 001581 ( Main/Exploration ); précédent : 001580; suivant : 001582Selective Binding of Endostatin Peptide 4 to Recombinant VEGF Receptor 3 In Vitro.
Auteurs : Kyu-Yeon Han ; Michael Chang ; Hong-Yu Ying ; Hyun Lee ; Yu-Hui Huang ; Jin-Hong Chang [États-Unis] ; Dimitri T. AzarSource :
- Protein and peptide letters [ 1875-5305 ] ; 2015.
Descripteurs français
- KwdFr :
- Collagène de type XVIII, Données de séquences moléculaires, Endostatines (), Endostatines (métabolisme), Liaison aux protéines, Protéines recombinantes (), Protéines recombinantes (métabolisme), Récepteur-3 au facteur croissance endothéliale vasculaire (), Récepteur-3 au facteur croissance endothéliale vasculaire (métabolisme), Séquence d'acides aminés.
- MESH :
- métabolisme : Endostatines, Protéines recombinantes, Récepteur-3 au facteur croissance endothéliale vasculaire.
- Collagène de type XVIII, Données de séquences moléculaires, Endostatines, Liaison aux protéines, Protéines recombinantes, Récepteur-3 au facteur croissance endothéliale vasculaire, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Collagen Type XVIII, Endostatins (chemistry), Endostatins (metabolism), Molecular Sequence Data, Protein Binding, Recombinant Proteins (chemistry), Recombinant Proteins (metabolism), Vascular Endothelial Growth Factor Receptor-3 (chemistry), Vascular Endothelial Growth Factor Receptor-3 (metabolism).
- MESH :
- chemical , chemistry : Endostatins, Recombinant Proteins, Vascular Endothelial Growth Factor Receptor-3.
- chemical , metabolism : Endostatins, Recombinant Proteins, Vascular Endothelial Growth Factor Receptor-3.
- chemical : Collagen Type XVIII.
- Amino Acid Sequence, Molecular Sequence Data, Protein Binding.
Abstract
We previously reported that neostatin, a proteolytic fragment of collagen XVIII that includes endostatin, inhibits basic fibroblast growth factor-induced corneal angiogenesis and lymphangiogenesis. In experiments to determine which fragments in neostatin are responsible for binding to VEGF receptors (VEGFRs), we previously showed that a 28- mer sequence at the C-terminal of endostatin, known as endostatin peptide 9, preferentially binds VEGFR3-Fc over VEGFR1-Fc and VEGFR2-Fc. In the present study, we show that a different endostatin fragment, endostatin peptide 4 (26 mers long), also selectively binds VEGFR3-Fc and not VEGFR1-Fc or VEGFR2-Fc. From surface plasmon resonance data, the KD and Chi² (RU²) values for endostatin peptide 4 binding to VEGFR3-Fc are 5.72 × 10⁻⁸ M and 0.354, respectively. In conclusion, endostatin peptides 4 and 9 may be responsible for endostatin binding to VEGFR3-Fc, and this improved understanding of endostatin peptide binding to VEGFR3-Fc may support the development of therapeutics targeting lymphangiogenic processes.
DOI: 10.2174/0929866522666150907111953
PubMed: 26343062
Affiliations:
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Le document en format XML
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Taylor Street, Chicago, IL 60612, USA. changr@uic.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, 1855 W. Taylor Street, Chicago, IL 60612</wicri:regionArea><placeName><region type="state">Illinois</region><settlement type="city">Chicago</settlement></placeName><orgName type="university">Université de l'Illinois à Chicago</orgName></affiliation></author><author><name sortKey="Azar, Dimitri T" sort="Azar, Dimitri T" uniqKey="Azar D" first="Dimitri T" last="Azar">Dimitri T. Azar</name></author></analytic><series><title level="j">Protein and peptide letters</title><idno type="eISSN">1875-5305</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Collagen Type XVIII</term><term>Endostatins (chemistry)</term><term>Endostatins (metabolism)</term><term>Molecular Sequence Data</term><term>Protein Binding</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (metabolism)</term><term>Vascular Endothelial Growth Factor Receptor-3 (chemistry)</term><term>Vascular Endothelial Growth Factor Receptor-3 (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Collagène de type XVIII</term><term>Données de séquences moléculaires</term><term>Endostatines ()</term><term>Endostatines (métabolisme)</term><term>Liaison aux protéines</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (métabolisme)</term><term>Récepteur-3 au facteur croissance endothéliale vasculaire ()</term><term>Récepteur-3 au facteur croissance endothéliale vasculaire (métabolisme)</term><term>Séquence d'acides aminés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Endostatins</term><term>Recombinant Proteins</term><term>Vascular Endothelial Growth Factor Receptor-3</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Endostatins</term><term>Recombinant Proteins</term><term>Vascular Endothelial Growth Factor Receptor-3</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Collagen Type XVIII</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Endostatines</term><term>Protéines recombinantes</term><term>Récepteur-3 au facteur croissance endothéliale vasculaire</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Molecular Sequence Data</term><term>Protein Binding</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Collagène de type XVIII</term><term>Données de séquences moléculaires</term><term>Endostatines</term><term>Liaison aux protéines</term><term>Protéines recombinantes</term><term>Récepteur-3 au facteur croissance endothéliale vasculaire</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We previously reported that neostatin, a proteolytic fragment of collagen XVIII that includes endostatin, inhibits basic fibroblast growth factor-induced corneal angiogenesis and lymphangiogenesis. In experiments to determine which fragments in neostatin are responsible for binding to VEGF receptors (VEGFRs), we previously showed that a 28- mer sequence at the C-terminal of endostatin, known as endostatin peptide 9, preferentially binds VEGFR3-Fc over VEGFR1-Fc and VEGFR2-Fc. In the present study, we show that a different endostatin fragment, endostatin peptide 4 (26 mers long), also selectively binds VEGFR3-Fc and not VEGFR1-Fc or VEGFR2-Fc. From surface plasmon resonance data, the KD and Chi² (RU²) values for endostatin peptide 4 binding to VEGFR3-Fc are 5.72 × 10⁻⁸ M and 0.354, respectively. In conclusion, endostatin peptides 4 and 9 may be responsible for endostatin binding to VEGFR3-Fc, and this improved understanding of endostatin peptide binding to VEGFR3-Fc may support the development of therapeutics targeting lymphangiogenic processes.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Illinois</li></region><settlement><li>Chicago</li></settlement><orgName><li>Université de l'Illinois à Chicago</li></orgName></list><tree><noCountry><name sortKey="Azar, Dimitri T" sort="Azar, Dimitri T" uniqKey="Azar D" first="Dimitri T" last="Azar">Dimitri T. Azar</name><name sortKey="Chang, Michael" sort="Chang, Michael" uniqKey="Chang M" first="Michael" last="Chang">Michael Chang</name><name sortKey="Han, Kyu Yeon" sort="Han, Kyu Yeon" uniqKey="Han K" first="Kyu-Yeon" last="Han">Kyu-Yeon Han</name><name sortKey="Huang, Yu Hui" sort="Huang, Yu Hui" uniqKey="Huang Y" first="Yu-Hui" last="Huang">Yu-Hui Huang</name><name sortKey="Lee, Hyun" sort="Lee, Hyun" uniqKey="Lee H" first="Hyun" last="Lee">Hyun Lee</name><name sortKey="Ying, Hong Yu" sort="Ying, Hong Yu" uniqKey="Ying H" first="Hong-Yu" last="Ying">Hong-Yu Ying</name></noCountry><country name="États-Unis"><region name="Illinois"><name sortKey="Chang, Jin Hong" sort="Chang, Jin Hong" uniqKey="Chang J" first="Jin-Hong" last="Chang">Jin-Hong Chang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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